Search results for " BBWV-2"

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Detection and identification Fabavirus species by one-step RT-PCR and multiplex RT-PCR

2014

The genus Fabavirus of the family Secoviridae comprises a group of poorly characterized viruses. To date, only five species have been described: Broad bean wilt virus 1 (BBWV-1), Broad bean wilt virus 2 (BBWV-2), Lamium mild mosaic virus (LMMV), Gentian mosaic virus (GeMV) and Cucurbit mild mosaic virus (CuMMV). The development is described of two RT-PCR procedures for the detection and identification of Fabavirus species: a one-step RT-PCR using a single pair of conserved primers for the detection of all fabaviruses, and a one-step multiplex RT-PCR using species-specific primers for the simultaneous detection and identification of the above-mentioned species of the genus Fabavirus. These m…

Time FactorsbiologyReverse Transcriptase Polymerase Chain ReactionBBWV-1 BBWV-2 GeMV CuMMV LMMV detection diagnosis multiplex RT-PCR conserved primersLamium mild mosaic virusSettore AGR/12 - Patologia VegetaleDetection Diagnosis Conserved primersbiology.organism_classificationSensitivity and SpecificityVirologyFabavirusBroad bean wilt virusReal-time polymerase chain reactionGenusVirologyPlant virusSecoviridaeRNA ViralIdentification (biology)MultiplexDNA PrimersPlant Diseases
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